Frozen Embryo Transfer

Bypass the adverse effects of stimulation drugs

Higher success rates compared to fresh cycles

Increased pregnancy rate per stimulated cycle

Used for extra embryos to avoid additional cycles

Used after biopsy for genetic testing, pending results

About the treatment

Frozen Embryo Transfer involves thawing cryopreserved embryos and transferring them to the female patient in a cycle that does not involve stimulation of the ovaries. The astonishing survival rate of vitrified embryos results in a high pregnancy outcome.

Our cryopreservation program has been utilising the vitrification method since 2007. We have an astonishing pregnancy rate when using frozen vs. fresh embryos.

Frozen Embryo Transfer

Vitrification is characterised as the second most ‘important invention’ in the IVF practice after Intracytoplasmic Sperm Injection (ICSI) due to its importance in the routine work of an IVF laboratory.

It is proven that in stimulated cycles endometrial receptivity is impaired due to stimulation drugs. Thus, a subsequent frozen embryo transfer could bypass this adverse effect and result in a successful pregnancy with similar or even higher pregnancy rate than fresh cycles. We are strong supporters of this idea, but more studies need to be conducted before we turn to a freezing all embryos strategy as a routine for all patients.

Vitrification of embryos at Pedieos IVF Center is used for

Storing extra embryos from an IVF cycle

Poor responders due to age higher than 39 or low AMH

With these patients, we take good quality embryos from several natural or minimal stimulation cycles and vitrify the embryos. Normally, we will have a maximum of 1 or 2 good quality embryos created from each cycle. When a sufficient number of good quality embryos have been created and vitrified, the frozen embryo transfer can be scheduled, thus maximizing the chance of pregnancy for these patients.

Patients with a risk of Ovarian Hyperstimulation Syndrome

Transfer will take place in a future non stimulated cycle with success rate similar to a fresh cycle.

Cases where embryos have to be biopsied for genetic testing

The embryos are cryopreserved following the biopsy so as to allow sufficient time for the genetic laboratory to return the results of the testing, which can take from a few days to a few weeks. When the results are in and there are ‘healthy’, transferable embryos, the female patient will be prepared for a frozen embryo transfer on her following menstrual cycle.

Our process


1. Medication

The female patient takes medication to prepare the uterine endometrium to accept the embryo. This medication is not the same as in the stimulation cycle for the oocyte collection.

Once the endometrium and the patient’s reproductive hormones are at an appropriate level, the embryos are thawed and transferred.

Embryo transfer treatment

2. Embryo transfer

Frozen embryo transfer can be done either on cleavage stage embryos (Day 3) or blastocyst embryos (Day 5).

Laser Assisted Hatching

3. Laser Assisted Hatching

Please note that we perform Laser Assisted Hatching on all of our warmed embryos before transfer free of charge. Studies confirmed that vitrified embryos have a thickened zona pellucida, so assisted hatching helps to overcome this potential problem by allowing the embryo to hatch on the zona pellucida and be implanted onto the uterus endometrium.

Contact us

Phone: +357 22670850
Give us a call or drop by anytime, we endeavour to answer all enquiries within 24 hours on business days.